Watcher Guardians (or Guardian Gargoyles: they are called both in Prima's Official Defiance Strategy Guide) appeared in Defiance, and served a similar function to the Soul Reaver 2 Sentries. They were proximity monitors; if Raziel or Kain ventured too close (in the Material Realm), they would lock their gaze on them, and trigger an automatic response to their approach. As the Watchers generally defended doorways, the common response was to close that door until Kain or Raziel moved out of range again. The first Watcher Guardians in Defiance were in the Cemetery.
Kain didn't have any means to sneak past the Watchers, so he was nearly always forced to find another route around them. Things were easier for Raziel - once he acquired the Dark Reaver, he could use its spell to make himself invisible so the Watchers could not track him.